The detection, identification, and quantification of biologically active compounds from complex matrices are challenging and time-consuming tasks for life science researchers. Advances in this field have generally been related to advances in analytical chemistry techniques. One major recent development is the emergence of metabolomics, which can be considered as the comprehensive analysis of low molecular weight metabolites (<1500 Da) in given organisms in different physiological states. The capacity of mass spectrometry to detect multiple compounds with high sensitivity makes it a key tool in metabolomics. At NPAC, we are actively contributing to advances in applied mass spectrometry through the development of novel methods for specific biological, chemical and environmental issues.